Triglycerides Reagent Kit (Glycerol-3-Phosphate-Oxidase Method) for Quantitative Determination
Sold by labgene bio-tech private limited · Manufacturer, Brand Owner, Exporter, Startup · Mahesana, Gujarat, India
Sold by labgene bio-tech private limited · Manufacturer, Brand Owner, Exporter, Startup · Mahesana, Gujarat, India
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Importers · Distributors · Wholesalers
labgene bio-tech private limited is a Manufacturer, Brand Owner, Exporter, Startup based in Mahesana Gujarat India.
Manufacturer, Brand Owner, Exporter, Startup · Mahesana, Gujarat, India
Labgene Biotech is a leading Indian manufacturer at the forefront of IVD product development. We operate an ISO 13485-certified, WHO-GMP, and USFDA-compliant facility, with all processes driven by hundreds of validated SOPs. Our R&D team has developed over 200 diagnostic tests, with 30+ currently in active use across chain laboratories, government platforms (GEM Portal), private distributors, and international export markets.
Registered address: Mahesana, India. Industry: Healthcare, Medical Supplies & Pharmaceuticals > Biotechnology.
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This in-vitro diagnostic (IVD) reagent kit is for the quantitative determination of triglycerides in human serum and plasma samples using a photometric system. The kit utilizes the enzymatic Glycerol-3-Phosphate-Oxidase method to provide accurate and reliable results. It is a vital diagnostic tool for assessing metabolic disorders and cardiovascular risk, as abnormal triglyceride levels are associated with various diseases.
Triglycerides are fatty acid esters of glycerol synthesized by liver cells. They serve as a primary energy source for the body and are transported by lipoproteins. Abnormal triglyceride levels are a significant risk factor for coronary artery disease and peripheral vascular disease. Increased concentrations can also be found in conditions such as diabetes, liver disease, hypothyroidism, and nephritic syndrome. Conversely, decreased concentrations may indicate malnutrition or hyperthyroidism.
This reagent kit employs a robust enzymatic method based on Glycerol-3-Phosphate-Oxidase (GPO). The determination of triglycerides involves a series of enzymatic reactions: first, lipoprotein lipase splits triglycerides into glycerol and fatty acids. The glycerol is then phosphorylated by glycerokinase to glycerol-3-phosphate. In the next step, GPO oxidizes glycerol-3-phosphate, producing hydrogen peroxide (H2 O2 ). Finally, the H2 O2 reacts with 4-aminoantipyrine and 4-chlorophenol, catalyzed by peroxidase, to form a colored quinoneimine indicator. The photometric measurement of this colored compound is directly proportional to the triglyceride concentration in the sample. This enzymatic, endpoint method provides high accuracy and ease of use for clinical laboratories, hospitals, and diagnostic centers.
Description provided by labgene bio-tech private limited.
| Detection Principle | Enzymatic photometric measurement of quinoneimine indicator |
|---|---|
| Sample Volume Required | Typically 10-20 µL serum or plasma |
| Reaction Time | Approximately 10-15 minutes |
| Storage Conditions | Store reagent at 2-8°C, avoid freezing |
| Shelf Life | Usually 12 months from manufacturing date |
| Operating Temperature | 28-37°C optimal assay temperature |
| Interferences | Minimal interference with hemolysis, lipemia controlled in protocol |
| Measurement Wavelength | Approximately 500 nm |
| Packaging | Reagent kit containing enzyme reagent, buffer, and chromogen |
| Compatibility | Suitable for automated analyzers and manual photometers |
| Category | In-vitro Diagnostic Reagents > Lipid Profile Reagents > Triglyceride Reagent Kits |
|---|---|
| Primary application | Wholesale, distribution, and international sourcing |
| Brand | labgene |
| Product type | Product |
| Lead time | On request |
|---|---|
| Packaging | Reagent kit containing enzyme reagent, buffer, and chromogen |
| Storage condition | As recommended by supplier |
| Origin | India |
| Form | As listed by supplier |
| Minimum order | 100 Numbers |
|---|---|
| Lead time | On request |
| Payment terms | As per negotiated commercial agreement. |
| Checkout type | enquiry |
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