SGPT/ALAT Reagent Kit (IFCC Modified Method) for Quantitative Determination

Alanine aminotransferase (ALAT), commonly known as SGPT, is an enzyme primarily located in the liver and kidneys. A low level of ALAT is normally present in the serum. However, increased ALAT levels are a significant indicator of liver damage and are used to screen for and monitor liver disease. ALAT is often measured concurrently with ASAT (Aspartate Aminotransferase) as part of a liver function panel to pinpoint the source of organ damage.

This reagent kit uses a kinetic enzymatic method modified to be suitable for photometric systems. The principle involves the enzyme ALAT catalyzing the transfer of an amino group from L-Alanine to 2-Oxoglutarate, producing L-Glutamate and Pyruvate. In a subsequent reaction, the pyruvate formed is consumed by lactate dehydrogenase (LDH) along with NADH (Nicotinamide Adenine Dinucleotide) to form L-Lactate and NAD+. The rate of NADH consumption is measured photometrically, which is directly proportional to the ALAT concentration in the sample. This robust and precise methodology provides rapid, accurate, and reliable results, making the kit an essential tool for hospitals, clinics, and diagnostic laboratories involved in liver function assessment.