Bilirubin Reagent Kit (Total & Direct) for Quantitative Determination

Bilirubin is a waste product of hemoglobin breakdown, with 80-85% of it derived from aging red blood cells. This bilirubin is transported to the liver, where it is conjugated to increase solubility before being excreted. Unconjugated bilirubin levels increase in conditions like hemolytic anemias and disorders of bilirubin metabolism, such as Gilbert's syndrome or Crigler-Najjar syndrome. A significant increase in conjugated bilirubin, with a minor increase in unconjugated bilirubin, indicates reduced excretion due to hepatocellular damage or bile flow obstruction.

This kit utilizes the Diazo with Sulphanilic Acid method to differentiate between Total and Direct (conjugated) Bilirubin. In the presence of an accelerator (cetrimide), both conjugated and unconjugated bilirubin react with diazotized sulfanilic acid to form azobilirubin, which represents Total Bilirubin. In the absence of the accelerator, only conjugated bilirubin reacts, measuring Direct Bilirubin. The resulting increase in absorbance at 546 nm is proportional to the bilirubin concentration. This comprehensive and precise method makes the kit highly useful for clinical professionals in hospitals and diagnostic laboratories to accurately assess liver function and diagnose a range of related conditions.