Cholesterol Reagent Kit (CHOD-POD Method) for Quantitative Determination
Sold by labgene bio-tech private limited · Manufacturer, Brand Owner, Exporter, Startup · Mahesana, Gujarat, India
Sold by labgene bio-tech private limited · Manufacturer, Brand Owner, Exporter, Startup · Mahesana, Gujarat, India
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Importers · Distributors · Wholesalers
labgene bio-tech private limited is a Manufacturer, Brand Owner, Exporter, Startup based in Mahesana Gujarat India.
Manufacturer, Brand Owner, Exporter, Startup · Mahesana, Gujarat, India
Labgene Biotech is a leading Indian manufacturer at the forefront of IVD product development. We operate an ISO 13485-certified, WHO-GMP, and USFDA-compliant facility, with all processes driven by hundreds of validated SOPs. Our R&D team has developed over 200 diagnostic tests, with 30+ currently in active use across chain laboratories, government platforms (GEM Portal), private distributors, and international export markets.
Registered address: Mahesana, India. Industry: Healthcare, Medical Supplies & Pharmaceuticals > Biotechnology.
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This is an in-vitro diagnostic (IVD) reagent kit designed for the quantitative determination of total cholesterol in human serum and plasma samples. It utilizes the enzymatic photometric CHOD-POD method to provide accurate and reproducible results. The kit is an essential diagnostic tool for assessing cardiovascular risk, as elevated cholesterol levels are a key indicator of coronary heart disease.
Cholesterol is a crucial component of cell membranes and a precursor for steroidal hormones and bile acids. It is transported in the blood by lipoproteins, including HDL and LDL. High levels of LDL are associated with the transport of cholesterol to peripheral cells, while HDL is responsible for cholesterol uptake from cells. An increase in total cholesterol concentration can indicate a risk of coronary heart disease.
The Cholesterol Reagent Kit employs the well-established CHOD-POD (Cholesterol Oxidase-Peroxidase) enzymatic method for precise and reliable results. The process involves two key steps : first, cholesterol esters are hydrolyzed by cholesterol esterase (CHE) into free cholesterol and fatty acids. Second, cholesterol oxidase (CHOD) oxidizes the free cholesterol, producing hydrogen peroxide (H2 O2). In a subsequent Trinder's reaction, peroxidase (POD) catalyzes the reaction between H2 O2, 4-aminoantipyrine, and phenol to form a colored quinoneimine. The intensity of this color is measured photometrically and is directly proportional to the total cholesterol concentration in the sample. This enzymatic method ensures high accuracy and specificity, making the kit an indispensable asset for clinical laboratories and hospitals for routine lipid profile testing and cardiovascular risk assessment.
Description provided by labgene bio-tech private limited.
| Assay Type | Enzymatic Colorimetric |
|---|---|
| Detection Method | Photometry |
| Sample Volume | Typically 10-100 µL per test depending on lab protocols |
| Reaction Temperature | Room temperature or 37°C incubation depending on protocol |
| Reagent Stability | Stable under recommended storage conditions (check kit label) |
| Storage Conditions | Store at 2-8°C, avoid freezing |
| Test Time | Approximate assay time 5-15 minutes |
| Measurement Wavelength | Approximately 500 nm (depends on photometer calibration) |
| Cholesterol Ester Hydrolysis | Catalyzed by cholesterol esterase present in kit |
| Enzymatic Oxidation | Cholesterol oxidase oxidizes cholesterol releasing H2O2 |
| Category | Laboratory & Scientific Supplies > Clinical Diagnostics > Reagent Kits > Cholesterol Test Kits |
|---|---|
| Primary application | Wholesale, distribution, and international sourcing |
| Brand | Not specified |
| Product type | Product |
| Lead time | On request |
|---|---|
| Packaging | As per buyer requirement |
| Storage condition | As recommended by supplier |
| Origin | India |
| Form | As listed by supplier |
| Minimum order | 100 Numbers |
|---|---|
| Lead time | On request |
| Payment terms | As per negotiated commercial agreement. |
| Checkout type | enquiry |
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