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Molecular Biology and Biotechnology: Microbial Methods (Hardback, Manoj V. Parakhia, Rukam S. Toma, Sunil V. Patel & B.A.Golakiya)

Name: Molecular Biology and Biotechnology: Microbial Methods (Hardback, Manoj V. Parakhia, Rukam S. Toma, Sunil V. Patel & B.A.Golakiya)
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Overview

Microorganisms, despite their small size, possess intricate makeups at the molecular level. The techniques detailed in Molecular Biology and Biotechnology encompass the majority of laboratory procedures and practical applications essential for both research and instruction in this field. This comprehensive text has been divided into 370 sections, each consisting of 36 subsections, followed by a thorough glossary. It serves as a valuable resource for microbiology researchers, educators, and students alike.

Product Description

1. Laboratory safety rules. 2. Milestones in DNA history and biotechnology. 3. Contribution in development of DNA and biotechnology. 4. Equipments required in a molecular marker laboratory. 5. Basic calculations used in microbial biotechnology. 6. Basic principals in preparation of reagents required in microbial biotechnology. 7. Isolation of bacterial genomic DNA. 8. Isolation of RNA from yeast. 9. Nucleotide composition of RNA. 10. DNA melting point determination. 11. Quantitative determination of DNA by DPA method and spectroscopic method. 12. Estimation of RNA by Orcinol method. 13. Electrophoresis. 14. SDS-PAGE electrophoresis. 15. Polymerase chain reaction. 16. Restriction enzyme digestion. 17. Extraction of DNA fragments from Agarose Gel. 18. Isolation the Mammalian DNA from whole blood. 19. Isolation of Plasmid DNA from bacteria. 20. Random Amplified Polymorphic DNA (RAPD). 21. Amplified Fragment Leanth Polymorphism (AFLP). 22. Southern blotting. 23. Ligation of insert DNA to Vector DNA. 24. Bacterial transformation. 25. Detection of plasmid by transformation. 26. Conjugation in E.Coli by plate method. 27. Isolation of Lactose non fermentor mutants of E.Coli by Physical mutagenesis. 28. Isolation of auxotrophic mutant by chemical (5 Bu) mutagenesis. 29. Mutagenesis (direct selection). 30. Mutagenesis (indirect selection). 31. Agrobacterium mediated gene transfer from bacteria to plant. 32. Western blotting. 33. Northern blotting. 34. PCR-sequencing. 35. Overview of metagenomics. 36. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.

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Specifications

Authors	Manoj V. Parakhia, Rukam S. Toma, Sunil V. Patel, B.A.Golakiya
Format	Hardback
Sections	370
Subsections per Section	36
Glossary	Yes
Publisher	Not specified
ISBN	Not provided

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Category	books and stationery > education supplies > teaching resources > medical science
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Buyer questions

Frequently asked by buyers

Common questions B2B buyers ask before sourcing Molecular Biology and Biotechnology: Microbial Methods (Hardback, Manoj V. Parakhia, Rukam S. Toma, Sunil V. Patel & B.A.Golakiya). Answers compiled from verified sellers' commercial terms and platform data.

Q: What topics does this book cover regarding laboratory techniques?

A: The book covers a broad range of laboratory techniques including DNA and RNA isolation, electrophoresis methods, PCR, plasmid isolation, various blotting techniques, and more.

Q: Is there a focus on safety in the laboratory protocols outlined in this book?

A: Yes, the book includes essential laboratory safety rules to ensure safe practices during microbiological procedures.

Q: Who is the intended audience for this book?

A: This book is intended for microbiology researchers, educators, and students seeking a comprehensive resource on microbial methods.

Q: Does the book provide calculations used in microbial biotechnology?

A: Absolutely, it includes basic calculations that are essential for practical applications in microbial biotechnology.

Q: Can this book be used as a textbook for academic courses?

A: Yes, its structured approach and comprehensive coverage make it suitable for use as a textbook in microbiology and biotechnology courses.

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