Molecular Biology and Biotechnology: Microbial Methods (Hardback, Manoj V. Parakhia, Rukam S. Toma, Sunil V. Patel & B.A.Golakiya)

Name: Molecular Biology and Biotechnology: Microbial Methods (Hardback, Manoj V. Parakhia, Rukam S. Toma, Sunil V. Patel & B.A.Golakiya)
Brand: NIPA
SKU: 8449289
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Molecular Biology and Biotechnology: Microbial Methods is an essential hardback resource for anyone involved in microbiology research, education, or practical applications. Authored by Manoj V. Parakhia, Rukam S. Toma, Sunil V. Patel & B.A.Golakiya, this comprehensive text dives deep into intricate microbial methods, spanning across 370 comprehensive sections that cover 36 detailed subsections each. It encapsulates vital laboratory safety protocols, milestones in DNA history, foundational calculations in microbial biotechnology, as well as elaborate instructions for techniques like PCR, plasmid isolation, and various blotting methods. With a clear glossary at the end, this book serves not only as a manual for experienced researchers but also as an educational tool for students, bridging the gap between theory and practical laboratory experience.

Key Features

Features	Description
Comprehensive Coverage	370 sections divided into 36 subsections each, ensuring thorough exploration of microbial methods.
In-depth Laboratory Protocols	Detailed instructions on essential laboratory techniques including DNA/RNA isolation, electrophoresis, and PCR.
Glossary & References	Extensive glossary available at the end for clear understanding of terms used.
Educational Resource	Designed for both researchers and students, providing practical applications and theoretical insights.
Safety Guidelines	Includes guidelines on laboratory safety rules, essential for any microbiological practice.

Attributes	Description
Authors	Manoj V. Parakhia, Rukam S. Toma, Sunil V. Patel, B.A.Golakiya
Format	Hardback
Sections	370
Subsections per Section	36
Glossary	Yes
Publisher	Not specified
ISBN	Not provided

Key Words

*Disclaimer: This above description has been AI generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.

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Brand: NIPA

Country of Origin: 101

Microorganisms, despite their small size, possess intricate makeups at the molecular level. The techniques detailed in Molecular Biology and Biotechnology encompass the majority of laboratory procedures and practical applications essential for both research and instruction in this field. This comprehensive text has been divided into 370 sections, each consisting of 36 subsections, followed by a thorough glossary. It serves as a valuable resource for microbiology researchers, educators, and students alike.

1. Laboratory safety rules. 2. Milestones in DNA history and biotechnology. 3. Contribution in development of DNA and biotechnology. 4. Equipments required in a molecular marker laboratory. 5. Basic calculations used in microbial biotechnology. 6. Basic principals in preparation of reagents required in microbial biotechnology. 7. Isolation of bacterial genomic DNA. 8. Isolation of RNA from yeast. 9. Nucleotide composition of RNA. 10. DNA melting point determination. 11. Quantitative determination of DNA by DPA method and spectroscopic method. 12. Estimation of RNA by Orcinol method. 13. Electrophoresis. 14. SDS-PAGE electrophoresis. 15. Polymerase chain reaction. 16. Restriction enzyme digestion. 17. Extraction of DNA fragments from Agarose Gel. 18. Isolation the Mammalian DNA from whole blood. 19. Isolation of Plasmid DNA from bacteria. 20. Random Amplified Polymorphic DNA (RAPD). 21. Amplified Fragment Leanth Polymorphism (AFLP). 22. Southern blotting. 23. Ligation of insert DNA to Vector DNA. 24. Bacterial transformation. 25. Detection of plasmid by transformation. 26. Conjugation in E.Coli by plate method. 27. Isolation of Lactose non fermentor mutants of E.Coli by Physical mutagenesis. 28. Isolation of auxotrophic mutant by chemical (5 Bu) mutagenesis. 29. Mutagenesis (direct selection). 30. Mutagenesis (indirect selection). 31. Agrobacterium mediated gene transfer from bacteria to plant. 32. Western blotting. 33. Northern blotting. 34. PCR-sequencing. 35. Overview of metagenomics. 36. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.

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