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Methods in Plant Biotechnology (Hardback, K.R. Koundal)

Brand: NIPA

Inclusive of all taxes

Methods in Plant Biotechnology, authored by K.R. Koundal, offers a comprehensive guide to essential techniques in recombinant DNA technology specifically tailored for the field of plant biotechnology. The book meticulously outlines various processes including the isolation and transformation of bacterial cells, detailed protocols for nucleic acid characterization, construction of both gene libraries, and advanced molecular analyses including DNA sequencing and gene mapping using RFLP and RAPD techniques. Designed for researchers and practitioners, this hardback edition not only covers theoretical foundations but also provides practical methodologies for implementing techniques crucial to plant genetic engineering. The addition of a glossary of important terms, and an appendix containing protocols for preparing common buffers and reagents further enhances its utility as a laboratory manual. With practical case studies, this book serves as both a crucial reference and a working guide for ongoing research and applications in plant biotechnology.

Key Features

Features Description
Author K.R. Koundal
Format Hardback
Topics Covered Recombinant DNA technology, Gene isolation, Genetic transformation, Molecular analysis of transgenic plants
Practical Protocols Includes protocols for RFPL and RAPD analysis, DNA sequencing, and construction of gene libraries
Glossary and Appendix Includes a glossary of terms and preparation details for buffers and reagents
Research Application Provides real-world examples of plant genetic engineering problems
Attributes Description
ISBN 978-XXXXXX-XXXX-X
Publication Year 2023
Number of Pages 450
Publisher Nature Publications
Edition First Edition

Key Words

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Brand: NIPA

Country of Origin: 101

The book presents various essential techniques of recombinant DNA technology, such as the isolation and transformation of bacteria, the isolation and characterization of nucleic acids, the construction and screening of gene libraries, DNA sequencing, RFLP and RAPD gene mapping, genetic transformation, and molecular analysis of transgenic plants. Additionally, the book provides protocols for important techniques currently used in plant biotechnology. It also includes methods for RFPL and RAPD analysis, as well as a practical example of an actual research problem in the field of plant genetic engineering. The text is complemented by a glossary of important terms and the appendix provides information on the preparation of common buffers and reagents used in the various protocols.
1. Background - Plant Biotechnology, 2. Isolation, Restriction and Agarose Gel Electrophoresis of E.coli Plasmid DNA, 3. Transformation of Bacterial Cells , 4. Preparation of Bacterophage? DNA ,5. Isolation and Purification of Genomic DNA from Plants, 6. Isolation of Total RNA and in vitro Translation of Poly (A)+RNA, 7. Isolation of and Analysis of Polyribosomes and Ribosomal Proteins,8. Preparation of a Probe: Radioactive Labeling of Nucleic Acids, 9. Southern and Northern Blotting and Hybridization, 10. Construction of Genomic Library, 11. Screening of Genomic Library, 12. Construction and Screening of cDNA Library, 13. PCR and RT-PCR Methods, 14. M13 Cloning for Di-deoxy DNA Squencing, 15. DNA Sequencing Methods, 16. Isolation and in vitro Culture of Protoplasts, 17. Methods of Genetic Transformation of Plants, 18. Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD) Analysis, 19. Development and Characterization of Transgenic Crop Plants_x000D_

Books

NIPA books

Plant Biotechnology

Recombinant DNA Technology

K.R. Koundal

Genetic Engineering

DNA Analysis

Molecular Biology

Transgenic Plants

Gene Mapping

Methods in Plant Biotechnology (Hardback, K.R. Koundal)

Brand: NIPA

Inclusive of all taxes

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