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Methods in Plant Biotechnology (Hardback, K.R. Koundal)

Name: Methods in Plant Biotechnology (Hardback, K.R. Koundal)
Brand: nipa
SKU: 8447377
Price: 295 USD
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We at NIPA when look back at the journey that started 47 years ago, we feel humbled. Sh Shree Pal Jain, the Founder, started the ﬁrm with just one room, one table and a set of chairs; with his sheer dedication, hard work, commitment and to develop everlasting relationships, throughout these nearly ﬁve decades have developed a customer centric and value-based company.

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Overview

The book presents various essential techniques of recombinant DNA technology, such as the isolation and transformation of bacteria, the isolation and characterization of nucleic acids, the construction and screening of gene libraries, DNA sequencing, RFLP and RAPD gene mapping, genetic transformation, and molecular analysis of transgenic plants. Additionally, the book provides protocols for important techniques currently used in plant biotechnology. It also includes methods for RFPL and RAPD analysis, as well as a practical example of an actual research problem in the field of plant genetic engineering. The text is complemented by a glossary of important terms and the appendix provides information on the preparation of common buffers and reagents used in the various protocols.

Product Description

1. Background - Plant Biotechnology, 2. Isolation, Restriction and Agarose Gel Electrophoresis of E.coli Plasmid DNA, 3. Transformation of Bacterial Cells , 4. Preparation of Bacterophage? DNA ,5. Isolation and Purification of Genomic DNA from Plants, 6. Isolation of Total RNA and in vitro Translation of Poly (A)+RNA, 7. Isolation of and Analysis of Polyribosomes and Ribosomal Proteins,8. Preparation of a Probe: Radioactive Labeling of Nucleic Acids, 9. Southern and Northern Blotting and Hybridization, 10. Construction of Genomic Library, 11. Screening of Genomic Library, 12. Construction and Screening of cDNA Library, 13. PCR and RT-PCR Methods, 14. M13 Cloning for Di-deoxy DNA Squencing, 15. DNA Sequencing Methods, 16. Isolation and in vitro Culture of Protoplasts, 17. Methods of Genetic Transformation of Plants, 18. Restriction Fragment Length Polymorphism (RFLP) and Random Amplified Polymorphic DNA (RAPD) Analysis, 19. Development and Characterization of Transgenic Crop Plants_x000D_

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Specifications

ISBN	978-XXXXXX-XXXX-X
Publication Year	2023
Number of Pages	450
Publisher	Nature Publications
Edition	First Edition

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Category	books and stationery > books and publications > electronic publications
Primary application	Wholesale, distribution, and international sourcing
Brand	nipa
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Common questions B2B buyers ask before sourcing Methods in Plant Biotechnology (Hardback, K.R. Koundal). Answers compiled from verified sellers' commercial terms and platform data.

Q: What advanced techniques are discussed in this book for plant biotechnology?

A: The book discusses various advanced techniques including gene isolation, transformation of bacterial cells, PCR methods, Southern and Northern blotting, and gene library construction.

Q: Does this book provide practical protocols for laboratory methods?

A: Yes, it contains detailed practical protocols for a range of methodologies used in plant biotechnology, including RFPL and RAPD analysis.

Q: Who is the target audience for 'Methods in Plant Biotechnology'?

A: The target audience includes researchers, students, and professionals in the fields of plant biotechnology, molecular biology, and genetic engineering.

Q: What resources are included in this book to assist with laboratory work?

A: The book includes a glossary of important terms and an appendix with preparation instructions for common buffers and reagents used in experiments.

Q: How does this book address real-world research problems in plant biotechnology?

A: It provides practical examples and case studies that highlight actual research challenges in the field of plant genetic engineering.

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