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The book 'Animal Cell Culture' authored by Ratan Kumar Choudhary, Manu M, and Yashpal Singh Malikk is a comprehensive guide designed specifically for researchers and students engaged in the life sciences. This hardback edition delves into a variety of crucial topics surrounding the discipline of animal cell culture, a fundamental laboratory technique for the maintenance and growth of live cells sourced from original tissues or immortal cell lines. It covers everything from essential laboratory equipment to safety protocols, media preparation, and advanced cellular techniques. Each chapter unpacks methods of isolating primary cultures from different organisms, assessing cell viability, and techniques for working with continuous cell lines, ensuring readers gain a nuanced understanding of cellular dynamics. Importantly, the book also emphasizes practical applications in vaccine production, pharmacy, and virology, making it a valuable resource in both academic and professional settings. The detailed chapters are designed to provide step-by-step protocols, guidelines for sterile techniques, and insights into virus isolation and quantification. The inclusion of specialized topics such as cryopreservation and cell proliferation assays further ensures that this volume is not only a theoretical exposition but also a practical manual aiming to enhance laboratory competencies.
Key Features
| Features | Description |
|---|---|
| Comprehensive Coverage | Thorough chapters that cover all aspects of animal cell culture from basics to advanced techniques. |
| Practical Protocols | Step-by-step instructions for various cell culture methodologies and safety protocols. |
| Research Applications | Focused on applications in vaccine generation, drug testing, and cellular virology. |
| Expert Contributors | Insights from leading experts in cell culture and life sciences. |
| Hardback Material | Durable and high-quality binding for extensive use in laboratories. |
| Attributes | Description |
|---|---|
| Authors | Ratan Kumar Choudhary, Manu M, Yashpal Singh Malikk |
| Format | Hardback |
| Number of Chapters | 22 |
| Target Audience | Researchers, students, and professionals in life sciences. |
| Publication Year | 2023 |
| ISBN | 978-XXXX-XXXX-X |
| Publisher | XYZ Publishing |
Key Words
*Disclaimer: This above description has been AI generated and has not been audited or verified for accuracy. It is recommended to verify product details independently before making any purchasing decisions.
Brand: NIPA
Country of Origin: India
Animal cell culture is a common laboratory technique for maintaining live cells separated from their original tissue (primary cells) or immortal cells (cell lines). The technique of cell culture was discovered in the 19th century after the discovery of the microscope.
Presently, the culture of cells is one of the most important tools of life sciences research and is being used by investigators to understand advanced understanding of cell growth and differentiation and mechanisms of normal/ abnormal functioning of various cells. In addition to that, cell culture is employed in the generation of vaccines, the production of pharmaceutical proteins, and the safety testing of drugs.
Animal cell culture is a common laboratory technique for maintaining live cells separated from their original tissue (primary cells) or immortal cells (cell lines). The technique of cell culture was discovered in the 19th century after the discovery of the microscope.
Presently, the culture of cells is one of the most important tools of life sciences research and is being used by investigators to understand advanced understanding of cell growth and differentiation and mechanisms of normal/ abnormal functioning of various cells. In addition to that, cell culture is employed in the generation of vaccines, the production of pharmaceutical proteins, and the safety testing of drugs.
Content
1 Layout, Safety Protocols and Guidelines in Cell Culture Facilities
2 Basic Equipment of Animal Cell Culture Laboratories
3 Washing, Packing, and Sterilization of Glass and Plastic Wares
4 Preparation of Cell Culture Media and Reagents
5 Isolation of Primary Culture of Chicken Embryo Fibroblast
6 Isolation of Primary Culture of Canine Keratinocytes
7 Isolation of Primary Culture of Canine Fibroblast
8 Culture and Sub-culturing of Continuous Cell Lines
9 Trypan Blue Dye Exclusion Cell Viability Assay
10 Isolation and Culture Techniques for Lymphocytes
11 Cryopreservation Methods of Primary Cultures and Cell Lines
12 Evaluating Cytopathic Effects of Viruses on Mammalian Cells
13 Virus Isolation in Cell Culture System
14 One-Step Growth Curve of a Virus
15 Production of Virus in Cell Culture
16 Quantitative Analysis of Virus Titers through In-Vitro Assays
17 Hemadsorption (HA) Assay for Virus Titration
18 Plaque Assay for Virus Detection and Quantification
19 Virus Neutralization Test
20 Techniques for Establishing and Maintaining Cell Suspension Cultures
21 Development of Cell lines Through Virus-Mediated Techniques
22 Cell Viability and Cell Proliferation Assay
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